Method and special complex enzyme for hydrolyzing galactomannan (GM) to prepare small-molecule GM and galactomannan oligosaccharide (GMOS)
US11999984B2 · kind B2 · utility
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Key dates
| Filing date | May 6, 2021 |
| Grant date | Jun 4, 2024 |
| Priority date | — |
| Expiry date | May 6, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01078
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and special complex enzyme for hydrolyzing a galactomannan (GM) to prepare a small-molecule GM and a galactomannan oligosaccharide (GMOS) is provided. The method includes: conducting fermentation with microcrystalline cellulose (MCC) and melibiose as carbon sources and Trichoderma reesei (T. reesei) as an enzyme-producing strain to obtain a supernatant, which is a complex enzyme solution with enzymatic activities of β-mannanase and α-galactosidase; and directly using the complex enzyme solution for enzymatic hydrolysis of a GM as a substrate to prepare the small-molecule GM and the GMOS.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.