Methods for production and quantification of unique molecular identifier-labeled beads
US11999994B2 · kind B2 · utility
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Key dates
| Filing date | Jan 13, 2022 |
| Grant date | Jun 4, 2024 |
| Priority date | — |
| Expiry date | May 16, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/11
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein are compositions and methods for the production and quantification of barcoded or unique molecular identifier (UMI)-labeled substrates. In one aspect, the substrate is a bead comprising a template oligonucleotide that is elongated by successive extension reactions to provide a bead with an oligonucleotide comprising a plurality of barcodes and conserved anchor regions. Methods are also described for quantifying the amount of template oligonucleotide loaded onto the substrate and the products of the extension reaction after each round and after the final extension.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.