Engineered polymerases with reduced sequence-specific errors
US12006518B2 · kind B2 · utility
2Cited by
15References
21Claims
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Key dates
| Filing date | Jan 27, 2023 |
| Grant date | Jun 11, 2024 |
| Priority date | — |
| Expiry date | Jan 27, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.