Production of steviol glycosides through whole cell biotransformation of steviol glycoside intermediates
US12012626B2 · kind B2 · utility
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17Claims
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Key dates
| Filing date | Dec 9, 2021 |
| Grant date | Jun 18, 2024 |
| Priority date | — |
| Expiry date | Dec 9, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y504/02002
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.