Method for producing psicose 3-epimerase by high-density fermentation
US12012627B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 15, 2023 |
| Grant date | Jun 18, 2024 |
| Priority date | — |
| Expiry date | Jan 15, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y501/03
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the technical field of microbial fermentation engineering, and specifically to a method for producing psicose 3-epimerase by high-density fermentation. In view of the low expression level of psicose 3-epimerase and other problems existing in the current fermentation, by controlling the feeding rate in the fermentation process, improving the culture temperature in the middle and late stages of fermentation and other measures in the present invention, the OD value during the fermentation with recombinant Bacillus subtilis and the total enzyme activity of psicose 3-epimerase in the fermentation broth are significantly increased, the expression of psicose 3-epimerase is markedly improved, and the production cost of allulose is reduced. Therefore, the present invention has a very broad prospect of application in industry.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.