Method and kit for single cell protein expression profiling of floorplate mesencephalic dopaminergic progenitor cells

US12013388B2 · kind B2 · utility

Assignee

• MILTENYI BIOTEC B.V. & CO. KG · DE

Inventors

• Melanie Jungblut · Moitzfeld, DE
• Andreas Bosio · Cologne, DE
• Sebastian Knobel · Cologne, DE
• Agnete Kirkeby · Hellerup, DK
• Malin Pernilla Parmar · Lund, SE

Key dates

• Filing date: Jul 16, 2018
• Grant date: Jun 18, 2024
• Expiry date: Nov 12, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2501/60
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides an in-vitro method for analyzing a cell composition comprising human floorplate mesDA progenitor cells, the method comprising a) contacting the cells of said cell composition or the cells of a sample thereof with antigen binding molecules specific for the antigens FOXA2, OTX2, PAX6, and NKX6.1, thereby labeling the cells of said cell composition or of said sample, b) determining the percentage of said cells that are labelled with said antigen binding molecules for each of said antigens, and wherein the cells of said cell composition qualify as human floorplate mesDA progenitor cells if the protein expression profile of said cells is: 80-100% of said cells are positive for FOXA2, 80-100% of said cells are positive for OTX2, less than 10% of said cells are positive for PAX6, and less than 10% of said cells are positive for NKX6.1. A kit comprising said antigen binding molecules for use in said method is also provided.