High-throughput microfluidic chip having parallelized constrictions for perturbing cell membranes
US12018240B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 27, 2021 |
| Grant date | Jun 25, 2024 |
| Priority date | — |
| Expiry date | Jan 15, 2042 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/086
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A microfluidic chip for causing the delivery of a payload to a cell comprises a plurality of constrictions configured to allow a cell suspension to flow through one or more of the plurality of constrictions from a first fluid flow region to a second fluid flow region within the microfluidic chip, wherein a cross-sectional width of each of the plurality of constrictions is less than a diameter of cells in the cell suspension, such that membranes of the cells are perturbed when passing through the constrictions such that a payload is able to pass through the perturbed cell membranes, and wherein a quotient of a cross-sectional area over a cross-sectional perimeter of each of the plurality of constrictions is greater than greater than or equal to 0.5 μm.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.