Method for large-scale preparation of purified preparation of recombinant lentiviral vector at GMP grade
US12018293B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 28, 2019 |
| Grant date | Jun 25, 2024 |
| Priority date | — |
| Expiry date | Sep 8, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/8813
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.