Long chain dibasic acid with low content of long chain dibasic acid impurity of shorter carbon-chain and preparation method thereof
US12018311B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 26, 2022 |
| Grant date | Jun 25, 2024 |
| Priority date | — |
| Expiry date | Dec 29, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y103/03006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.