Method and device for digital PCR measurement
US12018319B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 20, 2019 |
| Grant date | Jun 25, 2024 |
| Priority date | — |
| Expiry date | Jul 20, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2563/107
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention addresses the problem of providing a novel digital PCR analysis method. One embodiment of the novel digital PCR analysis method is a DNA detection method including the steps of: partitioning a DNA solution containing a fluorescent-labeled probe or a DNA intercalator and a target DNA to be detected into a plurality of compartments; carrying out a nucleic acid amplification reaction in the compartments; measuring a fluorescence intensity in association with a temperature change; calculating a melting temperature of a DNA double strand based on a change in the fluorescence intensity in association with the temperature change; and calculating a ratio of a fluorescence intensity at a second temperature that is lower than a first temperature in association with the temperature change to that at the first temperature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.