Method for quantitatively detecting deletion of human CDKN2A gene copy, primers and use thereof
US12018335B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 16, 2019 |
| Grant date | Jun 25, 2024 |
| Priority date | — |
| Expiry date | May 16, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are a set of primer pair and probe, having the oligonucleotide sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3, and a set of primer pair and probe of reference sequence, having the oligonucleotide sequences as shown in SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6. Further provided is the use of the set of primer pair and probe for detecting the common deleted region “chr9:21970277-21985225,hg19”, of CDKN2A in the preparation of a kit for quantitatively detecting the deletion of a human CDKN2A gene copy in a DNA sample to be tested. The new method and the specific primer pair and probe can simply, conveniently and specifically detect the deletion of the CDKN2A gene copy in the sample, and have higher sensitivity than conventional detection methods for copy deletion.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.