Fast volumetric imaging system and process for fluorescent tissue structures and activities
US12020453B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 29, 2020 |
| Grant date | Jun 25, 2024 |
| Priority date | — |
| Expiry date | Jan 7, 2041 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/10056
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A microscopic technique for generating high-clarity, large volume 3D images of fluorescent tissue structure at subcellular resolution and capture transient activities. The technique includes capturing two orthogonal 2D projection of the sample volume by performing a projection scan with an excitation laser sweeping through the volume at up to 100 vps, tracking the scan depth using an electrically tuned lens to keep the emission image in focus and generate an xy plane volume projection image at the camera; and placing a PMT behind the excitation lens to collect emission passed through the excitation lens, wherein signals from the PMT form a focus scan projection at the yz plane; and then merging the xy and yz projections.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.