Method for extracting Lactarius Hatsudake Tanaka polysaccharide compound

US12023349B2 · kind B2 · utility

Assignee

• CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY · CN

Inventors

• Jiali Ren · Hunan, CN
• Qiao Yang · Fürth, DE
• Songlin Chang · Hunan, CN
• Yiming Tian · Hunan, CN
• Hui Zhang · Jinan, CN
• Yiming Zhou · Hunan, CN

Key dates

• Filing date: Aug 23, 2023
• Grant date: Jul 2, 2024
• Expiry date: Aug 23, 2043

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present disclosure provides a method for extracting a Lactarius hatsudake Tanaka polysaccharide compound, and relates to the field of active substance extraction. This method includes: carrying out freeze-drying, pulverization, degreasing, water extraction, deproteinization, alcohol precipitation, and resin adsorption in sequence on Lactarius hatsudake Tanaka fruiting bodies to obtain a Lactarius hatsudake Tanaka refined polysaccharide mixture; to the Lactarius hatsudake Tanaka refined polysaccharide mixture, adding anhydrous ethanol dropwise to perform alcohol precipitation purification, and gradually collecting precipitates, to obtain Lactarius hatsudake Tanaka polysaccharide-10/40/60/80 active ingredients; and performing preparation via liquid chromatography on the Lactarius hatsudake Tanaka polysaccharide-10/40/60/80 active ingredients respectively, followed by dialysis and post-treatment to obtain Lactarius hatsudake Tanaka polysaccharide LHP-1, Lactarius hatsudake Tanaka polysaccharide LHP-2, Lactarius hatsudake Tanaka polysaccharide LHP-3, Lactarius hatsudake Tanaka polysaccharide LHP-4, and Lactarius hatsudake Tanaka polysaccharide LHP-5.