Amplification reporter with base-pairing oligomers

US12024737B2 · kind B2 · utility

Assignee

• Bio-Rad Laboratories, Inc. · US

Inventors

• John Frederick Regan · San Mateo, US
• Dianna Maar · Störitzseestraße, US
• Dawne N. Shelton · San Ramon, US
• Tina C. Legler · Tracy, US
• Samantha Cooper · Berkeley, US
• Wei Yang · Edison, US
• Eli A. Hefner · Fairfield, US
• Niels Klitgord · Oakland, US

Key dates

• Filing date: Aug 6, 2021
• Grant date: Jul 2, 2024
• Expiry date: Oct 11, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6848
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Method of analysis for alleles of a target. In the method, a plurality of fluid volumes may be formed. Each fluid volume may contain a first primer pair to amplify a first allele of a target, a second primer pair to amplify a second allele of the target, a first reporter including a first photoluminophore and providing a primer of the first primer pair, and a second reporter including a second photoluminophore and providing a primer of the second primer pair. Each of the first and second reporters may include an oligomer having a quencher, and the oligomer may be configured to base-pair with the primer of the first primer pair and the primer of the second primer pair. The first and second alleles may be amplified using the first and second primer pairs. Photoluminescence may be detected. A level of each allele may be determined.