RNASE H2 mutants that reduce primer dimers and off-target amplification in RHPCR-based amplicon sequencing with high-fidelity DNA polymerases
US12024742B2 · kind B2 · utility
0Cited by
1References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 22, 2021 |
| Grant date | Jul 2, 2024 |
| Priority date | — |
| Expiry date | Dec 22, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention pertains to hybrid RNase H2 proteins that include fragments of amino acid sequences from Pyrococcus abyssi (P.a.), Thermococcus kodakarensis (T.kod), and Pyrococcus furiosus organisms, as well as methods of using the same to improve mismatch discrimination and activity in a high-fidelity DNA polymerase buffer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.