Biotin-specific monoclonal antibody and use thereof

US12025613B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Michael Gerg · Munich, DE
• Dieter Heindl · Munich, DE
• Lars Hillringhaus · Penzberg, DE
• Klaus Hirzel · Baierbrunn, DE
• Caroline Dorothea Hojer · Munich, DE
• Florian Huber · Herrsching am Ammersee, DE
• Hans-Peter Josel · Weilheim, DE
• Thomas Meier · Cherbourg, FR
• Michael Schraeml · Penzberg, DE
• Edgar Voss · Bichl, DE

Key dates

• Filing date: Dec 15, 2021
• Grant date: Jul 2, 2024
• Expiry date: Dec 15, 2041

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/92
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as discloed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin/biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.