Methods of improving protein titer in cell culture
US12031151B2 · kind B2 · utility
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Key dates
| Filing date | Jan 19, 2022 |
| Grant date | Jul 9, 2024 |
| Priority date | — |
| Expiry date | Jun 30, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2511/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of improving recombinant protein titer and cell titer in cell culture using cell culture media having reduced impurities are provided, and well as cell culture media having reduced impurities that can used for the production of a recombinant protein and cells with improved titer. The cell culture media having reduced impurities comprises a HEPES buffer, and the reduced impurities are HEPES related impurities. In certain aspects, methods and media improve protein titer, cell growth, and/or viable cell density.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.