OMPG variants

US12037366B2 · kind B2 · utility

Assignee

• Roche Sequencing Solutions, Inc. · US

Inventors

• Cynthia Cech · Newcastle, US
• Tim CRAIG · Mountain View, US
• Christos TZITZILONIS · Redwood City, US
• Alexander Hyun-min YANG · Mountain View, US
• Liv JENSEN · Palo Alto, US
• Charlotte Yang · Mountain View, US
• Corissa Harris · Mountain View, US
• Matthew DiPietro · Gilroy, US
• Dhruti Vasudev Dalal · Sunnyvale, US

Key dates

• Filing date: Aug 15, 2023
• Grant date: Jul 16, 2024
• Expiry date: Aug 15, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure provides variant OmpG polypeptides, compositions comprising the OmpG variant polypeptides, and methods for using the variant OmpG polypeptides as nanopores for determining the sequence of single stranded nucleic acids. The variant OmpG nanopores reduce the ionic current noise versus the parental OmpG polypeptide from which they are derived and thereby enable sequencing of polynucleotides with single nucleotide resolution. The reduced ionic current noise also provides for the use of these OmpG nanopore variants in other single molecule sensing applications, e.g., protein sequencing.