Recombinant Bacillus subtilis for increasing yield of menaquinone 7 and application thereof
US12037576B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 3, 2020 |
| Grant date | Jul 16, 2024 |
| Priority date | — |
| Expiry date | Nov 9, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y602/01005
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides a recombinant Bacillus subtilis for increasing the yield of menaquinone 7 (MK-7) and application thereof, and belongs to the field of genetic engineering. In the present disclosure, 14 recombinant strains BS1-BS14 are constructed through the modification of genes related to the biosynthetic pathway of MK-7 on a chromosome of Bacillus subtilis, wherein BS6-BS14 significantly increase the yield of the MK-7, reaching up to 33.5 mg/L, which is 3.53 times the yield of the original strain of wild-type Bacillus subtilis 168. The present disclosure further provides a method for modifying the MK-7 biosynthetic pathway in microorganisms to increase the yield of the MK-7, providing a theoretical basis for constructing a high-yielding strain of the MK-7.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.