Recombinant expression vector applicable to rapid screening for recombinant strain and application
US12037632B2 · kind B2 · utility
Inventors
Key dates
| Filing date | Jul 13, 2019 |
| Grant date | Jul 16, 2024 |
| Priority date | — |
| Expiry date | Dec 29, 2040 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to the field of genetic engineering, particularly to a recombinant expression vector for rapidly screening the high expression strains and a method for rapidly screening high expression strains. In the invention, an exogenous red fluorescent protein and Aspergillus fumigatus cell surface protein localization signal are fused and expressed, and the fusion gene (DsRed-AfMP1) is integrated into the genome of Trichoderma reesei, so as to construct a strain displaying red fuorescent protein on the surface of Trichoderma reesei. By sorting Trichoderma reesei strains with red fluorescent protein on the surface by flow cytometry, genes beneficial to the improvement of cellulase activity can be quickly isolated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.