Kits and methods for pedigree division and paternity testing of domestic pigs

US12037648B2 · kind B2 · utility

Assignee

• Anhui Agricultural University · CN

Inventors

• Yueyun Ding · Hefei, CN
• Xiaoling Ding · St. Petersburg, US
• Zongjun Yin · Hefei, CN
• Xudong Wu · Zhejiang, CN
• Xiaodong Zhang · Pittsburgh, US
• Xianrui Zheng · Hefei, CN
• Zijing Ling · Hefei, CN
• Qiong Chen · Singapore, SG
• Yinhui Hou · Hefei, CN
• Chengcheng Kong · Hefei, CN

Key dates

• Filing date: Jul 11, 2022
• Grant date: Jul 16, 2024
• Expiry date: Dec 7, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure belongs to the field of livestock molecular biotechnology, and provides kits and methods for pedigree division and paternity testing of domestic pigs. The kits and methods specifically select 14 SSR loci of domestic pigs, especially Anqing six-end-white pigs, and synthesize primers for corresponding loci. Through capillary electrophoresis detection of the ear tissue DNA of 98 Anqing six-end-white pigs, the count of effective alleles, heterozygosity, polymorphism information content, and genetic distance, and exclusion probability at each locus are calculated, the pedigree division of domestic pigs, especially Anqing six-end-white pigs, and paternity testing are conducted. The cumulative exclusion probability of 14 microsatellite loci is 99%. The 14 microsatellite loci selected are polymorphic in Anqing six-end-white pig population, which can be used as effective genetic markers in the production practice of domestic pigs, especially in the pedigree division and paternity testing of Anqing six-end-white pig population.