Simultaneous detection of protein isoforms and nucleic acids from low starting cell numbers

US12038407B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Amy E. Herr · Oakland, US
• Lin He · Singapore, SG
• Andrew J. Modzelewski · Berkeley, US
• Elisabet Rosàs-Canyelles · Berkeley, US

Key dates

• Filing date: May 24, 2021
• Grant date: Jul 16, 2024
• Expiry date: Apr 25, 2043

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Dual nucleic acid and protein isoform measurements are performed on low starting cell numbers (e.g. equivalent to the number of blastomeres composing early embryonic development stages (morula and blastocysts)), comprising integrating fractionation polyacrylamide gel electrophoresis (fPAGE) of 10-100 cells with off-chip analysis of nucleic acids in the nuclei.