Molecule counting of methylated cell-free DNA for treatment monitoring
US12043873B2 · kind B2 · utility
0Cited by
10References
30Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 21, 2023 |
| Grant date | Jul 23, 2024 |
| Priority date | — |
| Expiry date | Mar 21, 2043 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/154
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods to quantify methylation in a DNA sample include treating the sample to encode the presence or absence of DNA methylation, adding to the sample a set of synthetic molecules (e.g., quality control template (QCT) molecules), generating a co-amplification mixture, sequencing the co-amplification mixture, and determining a number of methylated molecules in the sample based on the number of methylated reads from the sample and a number of reads from the set of synthetic molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.