Method for extracting Gymnadenia conopsea and related Gymnadenia conopsea extract

US12048726B2 · kind B2 · utility

Assignee

• Shanghai Hope-Tec Biotechnology Inc. · CN

Inventors

• Xing Liu · Hefei, CN
• Jibin Wang · Shanghai, CN
• Haihua Chen · Shanghai, CN
• Zuokun Ding · Shanghai, CN
• Chuanhao Wang · 安丰镇, CN
• Jiwen Wang · Shanghai, CN

Key dates

• Filing date: Apr 1, 2020
• Grant date: Jul 30, 2024
• Expiry date: Aug 27, 2041

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present disclosure provides a method for extracting Gymnadenia conopsea(L.)R.Br., which includes the following steps: (1) the root tuber of Gymnadenia conopsea(L.)R.Br. is soaked in water so that it can be fully infiltrated until having been taken as a sample, no white core is observed; (2) the root tuber of Gymnadenia conopsea(L.)R.Br. is ultrafinely comminuted by wet method to obtain a dispersion slurry; (3) additional water is supplemented into the dispersion slurry to obtain diluted dispersion followed by heating and adding neutral protease, and then extraction is carried out through circulation and homogenization by the homogenization pump to obtain extracted material fluid; (4) heat preservation and enzyme inactivation; (5) coarse filtration to obtain a coarse filtrate; and (6) fine filtration to obtain a fine filtrate. The disclosure also provides a related extract of Gymnadenia conopsea(L)R.Br.