Methods and compositions for forming ligation products
US12049665B2 · kind B2 · utility
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124References
15Claims
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Assignee
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Key dates
| Filing date | Jun 11, 2019 |
| Grant date | Jul 30, 2024 |
| Priority date | — |
| Expiry date | Jun 15, 2041 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In some aspects, the present disclosure provides methods for forming ligation products comprising single-stranded polynucleotides without the need for enriching the single-stranded polynucleotides. Ligation products formed by various aspects of the present disclosure can be useful for various applications, including but not limited to sequence analysis. In some embodiments, the ligation products comprise cell-free polynucleotides. In some aspects, the present disclosure provides reaction mixtures, kits and complexes consistent with the methods herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.