Fluorescent probe for cyclooxygenase-2
US12053533B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 26, 2021 |
| Grant date | Aug 6, 2024 |
| Priority date | — |
| Expiry date | Jun 17, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Cyclooxygenase-2 (COX-2) over-expression is prominent in inflammatory diseases, neurodegenerative disorders, and cancer. Directly monitoring COX-2 activity within its native environment poses an exciting approach to account for and illuminate the effect of the local environments on protein activity. Herein, we report the development of CoxFluor, the first activity-based sensing approach for monitoring COX-2 within live cells with confocal microscopy and flow cytometry. CoxFluor strategically links a natural substrate with a dye precursor to engage both the cyclooxygenase and peroxidase activities of COX-2. This catalyzes the release of resorufin and the natural product, as supported by molecular dynamics and ensemble docking. CoxFluor enabled the detection of oxygen-dependent changes in COX-2 activity that are independent of protein expression within live macrophage cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.