Method of ctDNA library construction and sequencing data analysis for simultaneously detecting multiple common mutations in liver cancer
US12054710B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 11, 2019 |
| Grant date | Aug 6, 2024 |
| Priority date | — |
| Expiry date | Dec 7, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of ctDNA library construction and sequencing data analysis for simultaneously detecting multiple common mutations in liver cancer. The library construction method and sequencing data analysis process have the following advantages: 1. Simultaneous detection of multiple mutation forms in liver cancer without capturing; 2. Suitable for efficient capture of ultra-small target regions; 3. The library may support 10-20 tests; 4. Ligate the DNA barcode to the starting ctDNA molecule during the library construction process, and cooperate with the biological information analysis process to achieve high specific detection of low-frequency mutations in ctDNA; 5. The library is usable for PCR hot spots detection and sequencing by a capture method at the same time, the added DNA barcode may effectively filter out false positive mutations and achieve high-specificity sequencing based on duplex. The disclosure helps with early screening, disease tracking, efficacy evaluation, prognosis prediction and the like of liver cancer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.