High subzero cryopreservation

US12058996B2 · kind B2 · utility

Assignee

• The General Hospital Corporation · US

Inventors

• Mehmet Toner · Boston, US
• Mustafa Korkut Uygun · Newton, US
• Shannon N. Tessier · Framingham, US
• Shannon Stott · Stoneham, US
• Lindong Weng · Seattle, US
• Reinier Johan De Vries · Boston, US
• Casie A. Pendexter · Rockland, US
• Stephanie EJ Cronin · Boston, US

Key dates

• Filing date: Jun 14, 2018
• Grant date: Aug 13, 2024
• Expiry date: Jul 23, 2039

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A01N1/168
• WIPO field: Basic materials chemistry
• WIPO sector: Chemistry

Abstract

This disclosure is related to methods of preserving biological samples, organs, and organisms. This disclosure is based, at least in part, on the discovery that in the presence of heterogenous extracellular ice, endothelial cells have improved attachment and viability after preservation, and particularly by uniformly nucleating ice across the microvasculature using ice nucleating agents, the chance of intracelluar ice formation can be significantly reduced.