Method of preparing pH-dependent antibodies
US12060413B2 · kind B2 · utility
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33Claims
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Key dates
| Filing date | May 10, 2018 |
| Grant date | Aug 13, 2024 |
| Priority date | — |
| Expiry date | Apr 17, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/04
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods for preparing engineered antibodies exhibiting improved pH-dependent antigen binding are disclosed. The methods are based on introduction of histidine residues at a subset of defined amino acid positions within the antibody CDRs. The set of amino acid positions selected for histidine substitution is derived from a heat-map of histidine occurrence within the CDRs of functional antibodies from a natural antibody repertoire. The methods provide a simpler and less time-consuming approach to the identification of pH-dependent antibody variants.
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