Use of constitutively active variants of growth factor receptors as selection markers for the generation of stable producer cell lines

US12060561B2 · kind B2 · utility

Assignee

• CEVEC Pharmaceuticals GmbH · DE

Inventors

• Nicole Faust · Cologne, DE
• Silke Wissing · Cologne, DE
• Nikola Strempel · Cologne, DE

Key dates

• Filing date: Aug 1, 2018
• Grant date: Aug 13, 2024
• Expiry date: Nov 2, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2501/105
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to methods for the selection of a stable producer cell line expressing one or more protein(s) of interest (POIs), comprising the stable transfection of cells with a gene encoding a constitutively active variant of a growth factor receptor, respective methods for the expression of one or more POIs in such stable producer cell lines, and uses of a constitutively active variant of a growth factor receptor as selection marker in the cultivation of cells.