Direct detection of single molecules on microparticles

US12061200B2 · kind B2 · utility

Assignee

• Abbott Laboratories · US

Inventors

• Qiaoqiao Ruan · Kildeer, US
• Patrick J. Macdonald · Newcastle, US
• Kerry M. Swift · Moran Corners, US
• Sergey Y. Tetin · Moran Corners, US
• Brenda Calfin · Chicago, US
• Zhen Lin · Moran Corners, US
• Richard Haack · Skokie, US
• Mark R. Pope · Grayslake, US
• John Prostko · Kenosha, US
• Xiaoxing Qiu · Gurnee, US
• M. Felicia Bogdan · Gurnee, US

Key dates

• Filing date: Dec 27, 2019
• Grant date: Aug 13, 2024
• Expiry date: Apr 3, 2042

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2015/1014
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.