Organ-on-chip platforms with reduced fluid volume
US12065635B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 19, 2019 |
| Grant date | Aug 20, 2024 |
| Priority date | — |
| Expiry date | May 1, 2042 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/084
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Organ-on-chip platforms with reduced fluid volumes include circulating fluid volumes of below 1000 μL, preferably about 500 μL or less. The platforms are adjustable for culturing cells with varied oxygen demand at various seeding densities. The platforms include at least one lane, wherein each lane includes at least one cell culture well, at least one oxygenator for fluid oxygenation, and a pump system containing at least one pump per lane. The oxygenator may include a separate fluid path for oxygenating the fluid, which allows controlling and measuring the oxygen concentration in the fluid, shortening the diffusion length, and passively diffusing oxygen. Provided are also different configurations for the oxygenator, fluid circulation in the platforms, attachment means for securing scaffolds to culture wells, and pneumatic plates.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.