Methods of preparing nucleic acid libraries and compositions and kits for practicing the same
US12065644B2 · kind B2 · utility
0Cited by
2References
27Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 13, 2018 |
| Grant date | Aug 20, 2024 |
| Priority date | — |
| Expiry date | Mar 7, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2525/191
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of preparing nucleic acid libraries are provided. Aspects of the methods include producing one or more libraries, including e.g., expression libraries and/or immune cell receptor repertoire libraries, from double stranded complementary DNA (cDNA) generated through a template-switching reaction involving a RNA sample. In some aspects, the methods include preparing a library from a single cell and/or a library indexed at the single cell level. Compositions and kits for use in performing the methods are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.