B-family DNA polymerase variant and kit comprising the same
US12065697B2 · kind B2 · utility
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18Claims
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Key dates
| Filing date | Jun 28, 2022 |
| Grant date | Aug 20, 2024 |
| Priority date | — |
| Expiry date | Jun 28, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein is a specific B-family DNA polymerase variants that exhibit an improved incorporation of nucleotide analogues for synthesizing polynucleotides and sequencing the associated nucleic acid template. More particularly, the DNA sequencing-by-synthesis method can be efficiently performed by said B-family DNA polymerase variants with a normal nucleic acid template and reversible dye-terminator nucleotides to precisely determine the sequence of associated nucleic acid template.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.