Patent · US Active

Methods of measuring extracellular vesicles and nanoparticles in complex matrices by light scattering

US12066382B2 · kind B2 · utility

0Cited by
7References
20Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 21, 2019
Grant dateAug 20, 2024
Priority date
Expiry dateJul 29, 2039

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2015/0053
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Described herein are novel rapid and reliable methods of detection of extracellular vesicles and quantifying extracellular vesicle concentrations and absolute number from various sources, including raw cell harvest. The methods described herein comprise detection of light scattering of extracellular vesicles in biological samples. Extracellular vesicles analyzed by the methods of this application have a stereotypical elution profile distinct from known contaminants. The methods described herein are a significant improvement over the state of the art and fulfills an unmet need in the field of extracellular vesicle manufacturing and quality control.

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