Method of osteogenic differentiation in microfluidic tissue culture systems

US12070746B2 · kind B2 · utility

Assignees

• EMULATE, INC. · US
• Cedars Sinai Medical Center · US

Inventors

• Dan Gazit · Modiin-Maccabim-Reut, IL
• Gadi Pelled · Los Angeles, US
• Zulma Gazit · Los Angeles, US
• Dmitriy Sheyn · Los Angeles, US
• Christopher David Hinojosa · Cambridge, US
• Norman Wen · Dedham, US
• Geraldine Hamilton · Cambridge, US

Key dates

• Filing date: Apr 20, 2018
• Grant date: Aug 27, 2024
• Expiry date: Aug 20, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M41/40
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Microfluidic “organ-on-a-chip” devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.