Method for producing double stranded polynucleotides based on oligonucleotides with selected and different melting temperatures
US12071650B2 · kind B2 · utility
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Key dates
| Filing date | Mar 13, 2018 |
| Grant date | Aug 27, 2024 |
| Priority date | — |
| Expiry date | May 3, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2533/107
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for identifying a group of single-stranded oligonucleotides for self-assembly into a double-stranded polynucleotide, the group comprising a plurality of overlapping complementary oligonucleotides, wherein each overlap between complementary oligonucleotides is selected to have a melting temperature (Tm) that differs from the melting temperatures of all other overlapping complementary oligonucleotides in the group.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.