Methods of cell selection and modifying cell metabolism
US12077786B2 · kind B2 · utility
0Cited by
8References
10Claims
0Family size
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Key dates
| Filing date | Feb 1, 2019 |
| Grant date | Sep 3, 2024 |
| Priority date | — |
| Expiry date | Aug 26, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.