Patent · US Active

Temperature shift for high yield expression of polypeptides in yeast and other transformed cells

US12077794B2 · kind B2 · utility

0Cited by
4References
18Claims
0Family size

Assignee

Inventors

Key dates

Filing dateFeb 11, 2019
Grant dateSep 3, 2024
Priority date—
Expiry dateJun 19, 2039

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2523/00
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.