Multiplexable super-resolution force spectroscopy using ultrasound methods and magnetic detection
US12085534B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 6, 2019 |
| Grant date | Sep 10, 2024 |
| Priority date | — |
| Expiry date | Jan 11, 2042 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB06B2201/77
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of measuring dissociation of the biomolecular bonds in one or multiple sample wells using super-resolution force spectroscopy (SURFS). SURFS utilizes precise ultrasound radiation to exert an acoustic radiation force on the biomolecular bonds labeled with magnetic particles. The force-induced dissociation of the protein bonds labeled with magnetic particles may be measured as a reduced magnetic signal by a magnetic sensor. The force resolution allows for differentiating biomolecular bonds with an extremely high level of precision. The biomolecular bonds include protein-protein, protein-nucleic acid, nucleic acid-nucleic acid, small molecule-protein, and small molecule-nucleic acid interactions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.