Method for producing a cultured cell sheet for tissue regeneration
US12097302B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 26, 2022 |
| Grant date | Sep 24, 2024 |
| Priority date | — |
| Expiry date | Apr 26, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Provided is a cell sheet suitable for cartilage repair. The present invention provides a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the cell sheet is negative for immunostaining using an antibody against type II collagen. The present invention also provides a method for producing a cell sheet for cartilage repair, formed from a culture of cells derived from a cartilage tissue, and the method includes culturing cells derived from a cartilage tissue on a surface of a membrane, where a temperature-responsive polymer is immobilized on the surface, to give the cell sheet. The culturing is stopped before the cell sheet becomes positive for immunostaining using an antibody against type II collagen.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.