RNA fixation and detection in CLARITY-based hydrogel tissue
US12098418B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Jan 6, 2022 |
| Grant date | Sep 24, 2024 |
| Priority date | — |
| Expiry date | Oct 11, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/0024
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods, kits, and systems for fixation of RNA permitting its detection in intact tissue, such as, large volume of mammalian tissue are disclosed. The methods, kits, and systems util-ize carbodiimide-based chemistry to stably retain RNAs in tissue clarified using CLARITY. Also provided herein are methods, kits, and systems for detection of RNAs ire clarified tissue.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.