Determining linear and circular forms of circulating nucleic acids
US12098429B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 25, 2020 |
| Grant date | Sep 24, 2024 |
| Priority date | — |
| Expiry date | Dec 11, 2042 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/10
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Techniques are provided for analyzing circular DNA in a biological sample (e.g., including cell-free DNA, such as plasma). For example, to measure circular DNA, cleaving can be performed to linearize the circular DNA so that they may be sequenced. Example cleaving techniques include restriction enzymes and transposases. Then, one or more criteria can be used to identify linearized DNA molecules, e.g., so as to differentiate from linear DNA molecules. An example criterion is mapping a pair of reversed end sequences to a reference genome. Another example criterion is identification of a cutting tag, e.g., associated with a restriction enzyme or an adapter sequence added by a transposase. Once circular DNA molecules (e.g., eccDNA and circular mitochondrial DNA) are identified, they may be analyzed (e.g., to determine a count, size profile, and/or methylation) to measure a property of the biological sample, including genetic properties and level of a disease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.