Mutant of nitrile hydratase derived from Caldalkalibacillus thermarum

US12104189B2 · kind B2 · utility

Assignee

• Jiangnan University · CN

Inventors

• Zhemin Zhou · Wuxi, CN
• Zhongmei Liu · Wuxi, CN
• Ting Li · Nanhu, CN
• Dezhi Chen · Wuxi, CN
• Sailan Zhang · Wuxi, CN
• Li Zhou · Cambridge, US
• Wenjing Cui · Wuxi, CN
• Zhongyi Cheng · Hangzhou City, CN
• Junling Guo · Wuxi, CN

Key dates

• Filing date: Aug 11, 2021
• Grant date: Oct 1, 2024
• Expiry date: Dec 3, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y402/01084
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosure discloses a mutant of nitrile hydratase derived from Caldalkalibacillus thermarum, and belongs to the technical field of enzyme engineering. The nitrile hydratase mutant Cal. t Nhase-A20V provided by the disclosure has a half-life of about 10 min at 70° C., which does not change much compared with the thermal stability of the wild enzyme. The specific enzyme activity of the mutant Cal. t Nhase-A20V is 128% of that of the wild enzyme. At the same time, the mutant also has better tolerance to a substrate and a product, and the final yield of nicotinamide produced by whole-cell catalysis reaches 598 g/L. Therefore, the nitrile hydratase mutant Cal. t Nhase-A20V provided by the disclosure has good enzymatic properties and is beneficial to future industrial production.