Cofactor self-sufficient Escherichia coli and construction method and application thereof
US12104191B2 · kind B2 · utility
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Key dates
| Filing date | Aug 31, 2022 |
| Grant date | Oct 1, 2024 |
| Priority date | — |
| Expiry date | Aug 31, 2042 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/101
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A cofactor self-sufficient Escherichia coli and its construction method and application in the synthesis of L-glufosinate are provided. The present invention expresses a NADH kinase and key enzymes of the cofactor synthesis pathway in E. coli, and knocks out the genes of enzymes that catabolizes cofactor, and with the addition of co-metabolic intermediates during cell incubation, the intracellular NADP(H) concentration is increased by at least 50% and the catalytic activity of glufosinate dehydrogenase by 2-fold, resulting in a significant increase in the spatiotemporal yield of the glufosinate synthesis reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.