PCR primer pair and application thereof

US12104205B2 · kind B2 · utility

Inventors

• Lin Yang · Shanghai, CN
• Haojun Jiang · Lo Wu, CN
• Peng Zeng · 东风镇, CN
• Xuehan Zhuang · Lo Wu, CN
• Ya Gao · Hubei, CN
• Yanyan Zhang · Lo Wu, CN
• Hui Jiang · Lo Wu, CN
• Jing Guo · Beijing, CN
• Fang Chen · Lo Wu, CN
• Xun Xu · Shanghai, CN

Key dates

• Filing date: Jun 20, 2017
• Grant date: Oct 1, 2024
• Expiry date: May 25, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3′ end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.