LC-MS/MS method for measurement of Aloesin in rat plasma

US12105070B2 · kind B2 · utility

Assignee

• HAINAN MEDICAL UNIVERSITY · CN

Inventors

• Yinfeng Tan · Hainan, CN
• Hailong Li · Cincinnati, US
• Youbin Li · Hainan, CN
• Xuesong Wang · Shanghai, CN
• Xue Xiang Cui · Beijing, CN

Key dates

• Filing date: Mar 12, 2021
• Grant date: Oct 1, 2024
• Expiry date: Dec 11, 2042

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N30/7233
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for measuring aloesin in rat plasma includes: taking the to-be-detected plasma containing aloesin, adding the methanol solution containing the internal standard compound aloeresin D thereinto, performing vortex precipitation, centrifuging, and collecting supernatant as to-be-detected sample; setting liquid chromatogram conditions as follows: carrying out gradient elution by adopting a mobile phase A and a mobile phase B, wherein the mobile phase A is an aqueous solution containing 0.1-1.0 thousandth (v/v) of formic acid, the mobile phase B is methanol; Setting mass spectrometry conditions as follows: using electrospray ion source, using negative ion detection, spray voltage being 4500 V, spray temperature being 550° C.; adopting multiple reaction ion monitoring scan mode, wherein the ion pair used for the quantitative analysis of aloesin is m/z 393.1→272.9, and the ion pair for the internal standard compound aloeresin D is m/z 555.3→144.9. The method improves aloesin detection in plasma.