Methods and systems for processing polynucleotides

US12110549B2 · kind B2 · utility

Assignee

• 10x Genomics, Inc. · US

Inventors

• Phillip Belgrader · Severna Park, US
• Zachary Bent · Pleasanton, US
• Rajiv Bharadwaj · Pleasanton, US
• Vijay Kumar Sreenivasa Gopalan · Pleasanton, US
• Josephine Harada · San Francisco, US
• Christopher Hindson · Pleasanton, US
• Mohammad Rahimi Lenji · Pleasanton, US
• Michael Lucero · South San Francisco, US
• Geoffrey McDermott · Livermore, US
• Elliott Meer · Pleasanton, US
• Tarjei Sigurd Mikkelsen · Dublin, US
• Christopher Joachim O'Keeffe · Pleasanton, US
• Katherine Pfeiffer · Berkeley, US
• Andrew D. Price · Hayward, US
• Paul Ryvkin · San Jose, US
• Serge Saxonov · Oakland, US
• John R. Stuelpnagel · Lompoc, US
• Jessica Michele Terry · Pleasanton, US
• Tobias Daniel Wheeler · Alameda, US
• Indira Wu · Redwood City, US
• Solongo Batjargal Ziraldo · San Ramon, US
• Stephane Claude Boutet · Burlingame, US
• Sarah Taylor · Pleasanton, US
• Niranjan Srinivas · Dublin, US

Key dates

• Filing date: Aug 24, 2022
• Grant date: Oct 8, 2024
• Expiry date: Oct 6, 2042

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2563/179
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.