Multivalent binding composition for nucleic acid analysis

US12117438B2 · kind B2 · utility

Inventors

• Sinan ARSLAN · San Diego, US
• Molly He · San Diego, US
• Matthew KELLINGER · San Diego, US
• Jake LEVIEUX · San Diego, US
• Michael Previte · San Diego, US
• Junhua ZHAO · San Diego, US
• Su-Chun Zhang · Middleton, US

Key dates

• Filing date: Jul 22, 2020
• Grant date: Oct 15, 2024
• Expiry date: Aug 7, 2043

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/582
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.