Dendritic detergents for the analysis of proteins by mass spectrometry

US12122805B2 · kind B2 · utility

Assignees

• OXFORD UNIVERSITY INNOVATION LIMITED · GB
• FREIE UNIVERSITÄT BERLIN · DE

Inventors

• Carol V. Robinson · North Hinksey, GB
• Idlir Liko · North Hinksey, GB
• Hsin-Yung Yen · North Hinksey, GB
• Kevin Pagel · Berlin, DE
• Rainer Haag · Berlin, DE
• Svenja Christina Ehrmann · Berlin, DE
• Leonhard Hagen Urner · Berlin, DE

Key dates

• Filing date: Sep 4, 2019
• Grant date: Oct 22, 2024
• Expiry date: Jan 5, 2042

Classification

• Technology area (CPC H): Electricity
• CPC primary: H01J49/165
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method of detecting a protein by mass spectrometry comprises: providing a solution comprising a dendritic detergent and a protein; providing a mass spectrometer comprising a nanoelectrospray ionisation source, a mass analyser and a detector; vaporising the solution using the nanoelectrospray ionisation source; ionising the protein; resolving the ionised protein using the mass analyser; and detecting the resolved protein using the detector. A method of extracting a membrane protein from its native membrane comprises: providing a membrane protein in its native membrane; contacting the membrane protein with a dendritic detergent. Dendritic detergents for use in the methods are also provided.